Flow Cytometry Workshop

What You never wanted to know about Flow Cytometry

13th May - 15th May 2019

Munich

Location:  

Helmholtz Zentrum München 
Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) 
Ingolstädter Landstr. 1 
85764 Neuherberg

Lecture hall "Großer Hörsaal“, Building. 33 (Institut für Strahlenschutz),  2nd floor, Room 106.

Investigator:

Helmholtz Zentrum München – IMA

Technische Universität München – CyTUM

TRR 179 and VFDCy

 

Workshop Overview
Flow Cytometry is a powerful analytical tool to characterize cells or particles on a single event level. In parallel, many different markers can be identified and quantified. That allows for a detailed characterization of single cells and categorization of mixed cell populations including the detection of rare cell subsets.

Referees from across Europe who are experts in their fields will provide detailed insight into state of the art flow cytometry projects, new technologies and methodologies including flow cytometry of microparticles using violet sideward scatter (V-SSC) and multiplex marker analysis. 

The workshop is designed for researchers at any step of their career. Basic knowledge in flow cytometry is of advantage. There will be strong emphasis on practical training. The topics will cover the whole process from planning your experiment, instrument setup, experimental procedure and data analysis. 

Workshop Structure
On day 1, new technical developments, research projects and methods will be presented.
Day 2 will cover data handling including basic statistics as well as cutting-edge algorithms for mass data processing and gate-less population analysis. Emphasis is also placed on practical sessions in technical set-up, assay procedures and tips for cell sorting.
On day 3, you will have the opportunity to individually meet with the experts to perform your own experiment in different sessions or discuss different challenges in flow cytometry in wet lab sessions.

Workshop Registration
To participate in the workshop, registration is required. The workshop is limited to 100 participants (first come – first served). If you wish to meet with a specialist for your own experiment on day 3, please provide a short summary of your experiments and the material needed.  There is no charge to attend this event.

Workshop Organizers
Helmholtz Zentrum München – Immunoanalytics – Core Facility, Thomas Hofer & Elfriede Nößner
Technische Universität München – CyTUM Core Facility, Matthias Schiemann

 

Programme:

 Day 1     Monday 13th May 2019
 09.00 - 10.00 Registration  
 10.00 - 10.15 Welcome address Thomas Hofer and Matthias Schiemann
 10.15 - 10.50 Basics and new Developments in Flow Cytometry: from Photo Multiplier Tube (PMT) to Avalanche Photo Diode (APD) Achim Egner, BCI Krefeld
 10.50 - 11.15 A one-year exposome panel study: Relationship between airborne pollen, allergic symptoms and nasal immune reactions under natural exposure Stefanie Gilles, UnikaT Augsburg
 11.15 - 11.40 Optimal analysis of monocyte subpopulations Löms Ziegler-Heitrock, Monocytomics Research Herrsching
 11.40 - 12.05 Sensitive and specific immunodiagnosis of active Tuberculosis: The TAM-TB assay. Christof Geldmacher, LMU München
 12.05 - 12.30 Core Facility & Organization of "Q&A" Thomas Hofer and Matthias Schiemann
 12.30 - 13.30 Lunch Break  
 13.30 - 14.00 Dura Clone dry pre-formulated Antibody Panels for Clinical Trials and Rare Event Detection Andreas Wicovsky, BCI Krefeld
 14.00 - 14.30 Role of Extracellular Vesicles in Cancer Kerstin Stemmer, HMGU München
 14.30 - 15.00 Challenges and opportunities using fluorescent proteins in multicolor-panels Simon Grassmann, TUM München
 15.00 - 15.30 Coffee Break  
 15.30 - 16.15 What can go wrong when Extracellular Vesicles are measured by flow cytometry Andreas Spittler, Medizinische Universität Wien
 16.15 - 17.00 Your limitation is your imagination Alfonso Blanco, University College Dublin
 
 Day 2  Tuesday 14th May 2019
 09.00 - 09.45 Flow cytometry standardised - in research and in a regulated environment. Mathias Streitz, Charité Berlin
 09.45 - 10.10 Basic statistics in flow cytometry Christian Gieger, HMGU München
 10.10 - 10.35 How to cope with massive Flow Data: UMAP and modern machine learning algorithms for efficient analysis of cytometry data Maren Büttner, HMGU München
 10.35 - 11.00 Coffee Break  
 11.00 - 12.30 Practical Session on CytoFLEX LX Alfonso Blanco, University College Dublin
 12.30 - 13.30 Lunch break  
 13.30 - 14.15 From analysis to purification - Practical Side of Cell Sorting: Basic principles and technical background Immanuel Andrä,TUM München & Achim Egner, BCI Krefeld
 14.15 - 15.00 Practical Sessions Microparticles Andreas Spittler, Medizinische Universität Wien
 15.00 - 15.30 Coffee Break  
 15.30 - 17.00 Practical Session – instrument setup – plan your experiment Q&A after the talks Mathias Streitz, Charité Berlin

 

 Day 3:   Wednesday 15th May 2019

Opportunity to use Flow Cytometers for your own experiments

(Indicate on registration page your options.  2 x 3 sessions will run parallel - please select at least two options when registering).

Please Note: if you are attending Day 3 please let us know in advance if you would like to bring your own samples.

-  Split for practical demonstration (3 groups/rooms)

-  Practical demonstration

  • Spillover and compensation
  • Analysis of participants samples
  • Controls and experimental design
  • Micro particles
  • Flow facility management
  • Analysis of flow data

 

 09.30 - 12.00  Practical sessions in 3 groups/rooms  all tutors
 12.00 - 13.00  Lunch Break  
 13.00 - 15.30  Practical sessions in 3 groups/rooms  all tutors
 15.30 - 16.00  Coffee Break  
 16.00 - 16.30  Final discussion (all together) farewell  Thomas Hofer and Matthias Schiemann

 

How to get here:

https://www.mvv-muenchen.de/en/journey-planer/index.html

From the airport:

S1 to station Oberschleißheim, then Bus 295 to destination Hauptbahnhof (central station). Get off at the 4th station, Helmholtz Zentrum. Bus takes about 12 minutes.


Or: S1 to station Feldmoching, with taxi or U2 (direction Messestadt Ost) to station Am Hart. From there bus 294 (direction Garching-Hochbrück) or bus 295 (direction to Oberschleißheim) to station Helmholtz Zentrum.


Or: S8 to main railway station (Hauptbahnhof), from there U2 (direction Feldmoching) to station Am Hart, then bus 294 (direction Garching-Hochbrück) or bus 295 (direction Oberschleißheim) to station Helmholtz Zentrum.


Main railway station:
U2 (direction Feldmoching) to station Am Hart, then bus 294 (direction Garching-Hochbrück) or bus 295 (direction Oberschleißheim) to station Helmholtz-Zentrum.

Munich's public transport system link: 

https://www.mvv-muenchen.de/fileadmin/mediapool/03-Plaene_Bahnhoefe/Netzplaene/MVV_Netzplan_S_U_R.pdf

By Car:  Parking spaces are available in front of the main gate.  Please report to gate before entering.

For additional information please contact hofer@helmholz-muenchen.de

 

Acknowledgements
This workshop is generously supported by Beckman Coulter providing access to Flow Cytometers and reagents, the VFDCy (Verein zur Förderung der Durchflußzytometrie e.V.) and TRR 179 (Determinants and dynamics of elimination versus persistence of hepatitus virus infection).